ELISA - Wikipedia
The assay is a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the ligand to be measured. ELISA has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries.
5.7: Binding - Enzyme Linked Immunosorbant Assays (ELISAs)
2023年7月13日 · There are several variants of ELISAs, including the traditional ELISA, in which the antigen is bound or fixed to the surface of the solid support, or a sandwich ELISA, in which the antibody is bound to the surface. In the latter case, a second labeled antibody that binds to the antigen must bind at a different site (or epitope) on the antigen.
一文读懂酶联免疫吸附实验（ELISA） - 知乎专栏
酶联免疫吸附实验（Enzyme linked immunosorbent assay，ELISA）是将抗原或抗体结合在固相载体表面，利用抗原抗体的特异性结合以及抗体或者抗原上标记的酶催化特定底物发生显色反应，实现目标物检测的免疫分析方法，可测至皮摩尔（pmol）级别。. 技术原理. ELISA可 ...
The enzyme linked immunosorbent assay (ELISA) is a powerful method for detecting and quantifying a specific protein in a complex mixture. Originally described by Engvall and Perlmann (1971), the method enables analysis of protein samples immobilized in microplate wells using specific antibodies.
Overview of ELISA | Thermo Fisher Scientific - US
The enzyme linked immunosorbent assay (ELISA) is a powerful method for detecting and quantifying a specific protein in a complex mixture. Originally described by Engvall and Perlmann (1971), the method enables analysis of protein samples immobilized in microplate wells using specific antibodies.
Enzyme Linked Immunosorbent Assay - StatPearls - NCBI Bookshelf
2023年4月23日 · Enzyme-linked immunosorbent assay (ELISA) is a heterogeneous EIA technique used in clinical analyses. In this type of assay, one of the reaction components is nonspecifically adsorbed or covalently bound to the surface of a solid phase, such as a microtiter well, a magnetic particle, or a plastic bead.
A Guide to Simple and Informative Binding Assays - PMC
2010年12月1日 · This essay explains proven experimental strategies to maximize the information obtained from reagents painstakingly acquired by biologists for binding studies. The tools for powerful binding assays are available in most laboratories. The essay also points out features of well-designed assays, which greatly simplify analysis of binding data.
An ELISA Based Binding and Competition Method to Rapidly …
2016年3月14日 · This article describes two fast and reliable point-by-point protocols to investigate the ligand-receptor interaction based on an enzyme linked immunosorbent assay (ELISA): the direct ligand-receptor interaction assay (LRA) and the competition LRA.
Enzyme-Linked Immunosorbent Assay: Types and Applications
2023年2月17日 · Enzyme-linked immunosorbent assay (ELISA) is an immunological assay widely used in basic science research, clinical application studies, and diagnostics. The ELISA technique relies on the interaction between the antigen (i.e., the target protein) versus the primary...
Overview of Enzyme-Linked Immunosorbent Assay (ELISA)
An enzyme-linked immunosorbent assay (ELISA) is a qualitative or quantitative test that uses antibodies to bind and measure a molecule of interest. Similar to other immunoassays, both monoclonal and polyclonal antibodies can be utilized to identify the analyte (eg, peptides, proteins, antibodies, small molecules).