Cell signaling and transcriptional regulation of osteoblast lineage ...
2024年7月2日 · Ephrin signaling regulated osteoblast-specific gene expression mainly through RhoA. EphrinA2 binds with EphA2 and promotes RhoA activity, while EphrinB1/2 binds with EphB4 and inhibits RhoA...
EphrinB2 Signalling in Osteoblast Differentiation, Bone …
2015年10月5日 · This work shows that the interaction of EphrinB2 with the receptor EphB4 stimulates partial differentiation of osteoblasts, but their ability to promote bone mineralisation is defective. This was the first evidence of an ephrinB2/EphB4 checkpoint in …
EphrinB2 signaling in osteoblasts promotes bone mineralization by ...
Microcomputed tomography, histomorphometry, and mechanical testing of the mice lacking ephrinB2 in osteoblasts revealed a 2-fold delay in bone mineralization, a significant reduction in bone stiffness, and a 50% reduction in osteoblast differentiation induced by anabolic parathyroid hormone (PTH) treatment, compared to littermate sex- and age ...
Bidirectional ephrinB2-EphB4 signaling controls bone homeostasis
Here we show that osteoclasts express the NFATc1 target gene Efnb2 (encoding ephrinB2), while osteoblasts express the receptor EphB4, along with other ephrin-Eph family members. Using gain- and loss-of-function experiments, we demonstrate that reverse signaling through ephrinB2 into osteoclast precursors suppresses osteoclast differentiation by ...
Increased autophagy in EphrinB2-deficient osteocytes is ... - Nature
2019年7月31日 · The interconnected osteocyte network within the bone matrix differentiates from bone-forming osteoblasts; although osteoblast differentiation requires EphrinB2, osteocytes retain its...
Communication Between EphrinB2 and EphB4 Within the Osteoblast …
2009年1月1日 · Since clustered ephrinB2 treatment of mouse calvarial osteoblasts has been shown to enhance expression of genes associated with osteoblast differentiation, and mice overexpressing EphB4 have increased bone formation parameters, we used inhibitors of ephrinB2-EphB4 receptor interaction in in vitro experiments to determine whether, within a ...
EphrinB2–EphB4 Signaling in Neurooncological Disease - PMC
Bidirectional ephrin-B2–EphB4 signaling plays a crucial role in preserving the delicate balance between bone-resorbing osteoclasts and bone-forming osteoblasts [16,69]. It was found that ephrinB2 is expressed on osteoclasts, whereas EphB4, along with other Eph family members, was initially described on osteoblasts.
Ephrin B2/EphB4 Mediates the Actions of IGF-I Signaling in …
Ephrin B2/EphB4 mediates interactions among osteoblasts (OB), osteoclasts (OCL), and chondrocytes to regulate their differentiation. We investigated the role of ephrin B2/EphB4 signaling in mediating the anabolic effects of IGF1 and PTH on those cells and overall endochondral bone formation.
Cellular Processes by Which Osteoblasts and Osteocytes Control …
Ephrin-B2. Stage-specific EphrinB2 deletion differs in its effects on skeletal strength. Early-stage deletion in osteoblasts leads to osteoblast apoptosis, delayed initiation of mineralization, and increased bone flexibility. Deletion later in the lineage targeted to osteocytes leads to a brittle bone phenotyp ….
Frontiers | Eph-Ephrin Signaling Mediates Cross-Talk Within the …
2021年2月8日 · Currently known targets include IGF-1 and PTH, which interact with Eph-ephrin signaling during osteoblast and osteoclast formation and function; the communication with CXCL12 signaling during hematopoietic niche maintenance; or the interaction with integrin molecules during cell adhesion of endothelial cells or osteoclasts.