Fluorescence spectroscopy - Wikipedia
Fluorescence spectroscopy (also known as fluorimetry or spectrofluorometry) is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample.
《分析化学》（十一）—荧光分析法 - 知乎 - 知乎专栏
激光诱导荧光分析(laser-induced fluorometry)： 采用单色性极好，强度更大的激光作为光源的荧光分析法。 与一般光源荧光测定相比，灵敏度可提高2~10倍，甚至可进行单分子检测。
Fluorimetry: Principle, Instrumentation, Factors, Uses
2024年4月27日 · Fluorimetry is a scientific and analytical technique used to detect and measure the fluorescent light emitted by the sample (lying in the visible spectrum) with the aid of ultraviolet rays. Here, the wavelength of the emitted light by the substance is greater than the absorbed light. This phenomenon is called luminescence (cold light).
Fluorometry - an overview | ScienceDirect Topics
Fluorometry is superior to spectrophotometry in terms of sensitivity and specificity. In general, the sensitivity of fluorescence is 10–1000-fold higher in comparison to absorbance measurements. Fluorometry was introduced in immunological assays to improve immunoassay sensitivity.
Fluorimetry | Principle, Instrumentation and Applications
2019年3月26日 · Fluorimetry is a type of spectroscopy that measures the emitted radiation from a substance. This radiation is one that is emitted by the substance when the electrons transit from the excited state to the ground state. In UV-Visible spectroscopy, the excitation wavelength is measured. But, here, the emission wavelength is measured.
Fluorometer - Wikipedia
A fluorometer, fluorimeter or fluormeter is a device used to measure parameters of visible spectrum fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. [1] . These parameters are used to identify the presence and the amount of specific molecules in a medium.
Fluorescence Spectroscopy - an overview | ScienceDirect Topics
Fluorescence spectroscopy is an emission spectroscopic technique for analyzing a sample’s fluorescence properties. This tool can provide information at a nanoscopic level with exceptional sensitivity in the case of polymer nanocomposites. The fluorescence can be attributed to a polymer, a nanofiller, or both.
Fluorescence Spectrophotometer: Principle, Components, …
2024年1月20日 · A fluorescence spectrophotometer, often known as fluorimetry or spectrofluorometry, is a sophisticated branch of electromagnetic spectroscopy that analyzes the fluorescence generated by a specimen. This approach uses a specialized laser beam, primarily in the UV range, to activate the electrons in certain chemical compounds.
Principles and Theory of Fluorescence Spectroscopy - HORIBA
Fluorescence is a type of luminescence caused by photons exciting a molecule, raising it to an electronic excited state. It’s brought about by absorption of photons in the singlet ground state promoted to a singlet-excited state. As the excited molecule returns to ground state, emits a photon of lower energy, which corresponds to a longer ...
General. All fluorescence instruments contain three basic items: a source of light, a sample holder and a detector. In addition, to be of analytical use, the wavelength of incident radiation needs to be selectable and the detector signal capable of precise manipulation and presentation (Figure 5).