技术 | 检测葡萄糖摄取的方法比较 - Promega
Promega开发了一种生物发光葡萄糖摄取检测方法 Glucose Uptake-Glo™ Assay ，它基于与上述检测相同的原理：2DG6P 的积累和通过G6PDH的酶促作用进行检测 (3)。生物发光法具有以下许多优点：
Measurement of Glucose Uptake in Cultured Cells - PubMed
2015年12月8日 · Evaluation of glucose uptake is crucial in the study of numerous diseases and metabolic disorders such as myocardial ischemia, diabetes mellitus, and cancer. Detailed in this unit are laboratory methods for assessing glucose uptake into mammalian cells.
Recent Advances in In-Vitro Assays for Type 2 Diabetes Mellitus: …
2021年5月1日 · Glucose transporter 4 (GLUT4) is a glucose transporter, present in adipose tissue, muscle cells, and other tissue; it is responsible for insulin-dependent glucose uptake in these cells. There are various types of glucose uptake assays available that use glucose analog, either radiolabeled or non-radiolabeled [14, 15].
Glucose Uptake-Glo™ Assay_产品_Promega微网站-普洛麦格（北 …
Glucose Uptake-Glo™ Assay 是一种非放射性的、基于培养板的、均相生物发光检测系统，用于检测哺乳动物细胞的葡萄糖摄取。. 试剂盒原理是基于对2- 脱氧葡萄糖-6- 磷酸 (2DG6P) 的检测。. 向细胞加入2- 脱氧葡萄糖 (2DG) 后，2DG 被转运进入细胞并迅速发生磷酸化 ( 磷酸 ...
Bioluminescent-based imaging and quantification of glucose uptake …
2019年5月13日 · Here, we report the development of a bioluminescent glucose-uptake probe for real-time, non-invasive longitudinal imaging of glucose absorption both in vitro and in vivo.
Glucose Uptake Measurement and Response to Insulin Stimulation …
2017年6月25日 · Glucose uptake is a key biological measurement for testing activators or inhibitors on cell culture and how they impact glucose use, and the ability of the cell to respond to insulin. The method described here has been shown to be quick and reliable and has been widely used in many studies using primary myotubes from healthy subjects and/or ...
Glucose Uptake Assay试剂盒(Colorimetric) (ab136955) - Abcam …
Glucose Uptake Assay Kit (Colorimetric) (ab136955) is a highly sensitive and easy to use non-radioactive assay kit which can detect glucose uptake as low as 10 pmol/well in a variety of cell types.
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Post-click labeling enables highly accurate single cell analyses of ...
2024年4月16日 · For accurate single cell analyses of glucose uptake ex vivo and in vivo, the authors developed a method using glucose transporter-dependent import of clickable sugars, coupled with...
Measurement of glucose uptake in cultured cells - PubMed
The work is divided into four sections: (1) a brief overview of glucose uptake assays in cultured cells; (2) a method for measuring glucose uptake using radiolabeled 3-O-methylglucose; (3) a method for measuring glucose uptake using radiolabeled 2-deoxyglucose (2DG); and (4) an improved method for measuring 2DG-uptake using an enzymatic ...
A real-time method of imaging glucose uptake in single, living ...
2007年3月29日 · This protocol details a method for real-time monitoring of glucose uptake in single mammalian cells using a fluorescent D -glucose derivative, 2- [N -...
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