A protoplast-based transient gene expression assay for the ...
2024年5月9日 · Treatment with 100 mM H 2 O 2 killed all protoplasts within 5 min, while treatment with 25 mM H 2 O 2 could not discriminate the transgenes’ effects. H 2 O 2 treatment at 37.5–50 mM is the optimal condition.
Hydrogen peroxide positively regulates brassinosteroid signaling ...
2018年3月14日 · ABA treatment induces the production of H 2 O 2, which in turn activates the plasma membrane calcium channel and promotes the stomatal closure.
Protoplast: A Valuable Toolbox to Investigate Plant Stress …
2021年10月4日 · For instance, in Arabidopsis, the molecular probe ContPY1 was used to detect the intracellular accumulation of a ROS, hydrogen peroxide (H 2 O 2) in response to the elicitor COS-OGA. The comparison between protoplasts and cell suspensions evidenced the relative contribution of CW peroxidases and membrane dehydrogenases to H 2 O 2 production ...
ROS regulated reversible protein phase separation …
2021年2月25日 · Here, we reveal that developmentally produced H 2 O 2 in plant shoot apical meristem (SAM) triggers reversible protein phase separation of TERMINATING FLOWER (TMF), a transcription factor that...
Optimized protocols for protoplast isolation, transfection, and ...
2024年3月5日 · Here we highlight the current research advances in protoplast transformation and discuss how to optimize the procedures of protoplast isolation, transfection, and regeneration for efficient and reproducible CRISPR/Cas-based …
Isolation, Purification, and Application of Protoplasts and Transient ...
2023年11月29日 · In this review, we provide a comprehensive overview, including details on isolation procedures and influencing factors, purification and viability assessment methodologies, and the utilization of the protoplast transient expression system.
A Robotic Platform for High-throughput Protoplast Isolation and ...
In this work, a protocol has been developed for automation of protoplast isolation and transformation from a 'Bright Yellow' 2 (BY-2) tobacco suspension culture using a robotic platform. ... Over-expression of Trxo1 increases the viability of tobacco BY-2 cells under H2O2 treatment. Ann. ... Golgi stacks in tobacco BY-2 cells reveals similar ...
Specific localization and measurement of hydrogen peroxide in ...
The comparison of cell suspensions, protoplasts of cell suspensions and leaf protoplasts treated with different inhibitors gave indications on the potential sources of hydrogen peroxide in plant cells.
Exogenous H2O2-mediated changes in ROS content of …
Exogenous H2O2-mediated changes in ROS content of Arabidopsis protoplasts. (A) Fluorescence images of protoplasts with a scale of 25 μm; (B-D) fluorescence intensity gradients induced by H2O2...
Plant protoplasts in the age of synthetic biology
2023年5月23日 · Protoplasts have been extensively used for validating the mutagenesis efficiency of DNA-free gene editing protocols in which the CRISPR/Cas [clustered, regularly interspaced, short, palindromic repeats)/CRISPR-associated protein] machinery is delivered directly to the cells without DNA insertions (see Yue et al., 2021).
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