Immunofluorescence (IF) is a light microscopy-based technique that allows detection and localization of a wide variety of target biomolecules within a cell or tissue at a quantitative level. The technique utilizes the binding specificity of antibodies and antigens. [1]

2022年3月1日 · Immunofluorescence is a type of assay performed on biological samples to detect specific antigens in any biological specimen or sample and vice-versa. The specificity of antibodies to their antigen is the base for immunofluorescence.

Immunofluorescence (IF) is a technique that permits visualization of virtually many components in any given tissue or cell type. This broad capability is achieved through combinations of specific antibodies tagged with fluorophores.

Immunofluorescence assay is based on specific antibodies for detecting and visualizing particular proteins or antigens in biological samples using fluorescence microscopy. The immunofluorescence assay principle relies on the antibodies’ specificity for their target proteins.

2024年4月9日 · Immunofluorescence is a laboratory technique used to detect specific proteins or antigens in biological samples by using antibodies labeled with fluorescent molecules, allowing visualization under a fluorescence microscope.

2021年11月16日 · What is immunofluorescence? Generally speaking, IF is an application in which antibodies that have been joined to fluorescent molecules are applied to a cell or tissue sample where they bind to their targets, most commonly proteins.

2021年3月18日 · Immunofluorescence is a highly powerful microscope-based technique that is used both in pre-clinical research and clinical settings to detect the presence of specific target molecules. Image...

Immunofluorescence (IF) is an important immunochemical technique that allows for detection and localization of a wide variety of antigens in different types of tissues of various cell preparations. IF allows for excellent sensitivity and amplification of signal in comparison to immunohistochemistry, …

2020年12月16日 · What is immunofluorescence? Immunofluorescence is one of the techniques used within light microscopy, especially on microbiological samples. It uses a fluorescence microscope to observe antibodies, bonded to their antigens, with fluorescent dyes attached to specific biomolecule targets.

2018年5月24日 · Immunofluorescence (IF) is a histochemical laboratory staining technique that relies on antibodies–antigens interactions in tissue or body fluids [1, 2]. IF is broadly used in clinical immunology laboratories to help in the diagnosis of autoimmune diseases and in patients’ treatment and monitoring.

In the study of autoimmune diseases, the laboratory plays a very important role. We describe the immunofluorescence techniques (direct, indirect, complement-fixing, double) for determining the presence of autoantibodies and their role in the autoimmune endocrine diseases.

Immunofluorescence (IF) is a powerful approach for getting insight into cellular structures and processes using microscopy. Specific proteins can be assessed for their expression and location, making immunofluorescence indispensable for scientists to solve many cell biological questions.

2023年7月4日 · Immunofluorescent methods are a simple yet effective method for determining the presence of different biological molecules such as proteins, carbohydrates, and small molecules. Immunofluorescence methods offer enhanced sensitivity, and can simultaneously detect multiple epitopes (multiplexing).

Information and resources for immunofluorescence studies for all experience levels, including protocols, experimental design guidance, and instrument and reagent selection help.

Multiplex Immunohistochemistry/Immunofluorescence (mIHC/IF) technologies, which allow the simultaneous detection of multiple markers on a single tissue section, have been introduced and adopted in both research and clinical settings in response to …

Immunofluorescence (IF) uses antibodies and fluorescent detection to study the localization, relative expression, and activation states of target proteins in fixed cells or tissues. IF is widely used in diverse fields of study.

Immunofluorescence (IF) staining labels a specific target antigen with a fluorescent dye such as fluorescein isothiocyanate or cyanine. The fluorescent stain visualized under a fluorescent microscope allows for an assessment of the target molecule/protein distribution in the sample.

Immunofluorescence (IF) staining is a widely used technique in biological research and clinical diagnostics. IF utilizes fluorescent-labeled antibodies in order to detect specific target antigens. Followed by imaging, it is a very direct technique as you can actually see something.

2015年12月1日 · This unit describes the widely used and powerful technique of localization of proteins in cells by immunofluorescence. The location can be determined by double labeling with an antibody directed against a protein of known location.

2022年6月2日 · Here we introduce scission-accelerated fluorophore exchange (SAFE), a method for multiplexed temporospatial imaging of living cells with immunofluorescence.