自噬标志物—LC3检测要点 - 知乎 - 知乎专栏
自噬标志物—LC3检测要点. 细胞自噬（Autophagy）是一类细胞内物质降解的代谢过程。. 细胞内待降解的胞内物质首先被自噬小泡包裹，随后被溶酶体中的水解酶降解。. 微管相关蛋白1轻链3 (MAP1LC3)，是Atg8在哺乳动物中的同源物。. LC3/Atg8 被具有蛋白内切酶活性的Atg4 ...
LC3 and Autophagy - PubMed
Microtubule-associated protein 1A/1B-light chain 3 (LC3) is a soluble protein with a molecular mass of approximately 17 kDa that is distributed ubiquitously in mammalian tissues and cultured cells. During autophagy, autophagosomes engulf cytoplasmic components, including cytosolic proteins and organ ….
MAP1LC3B - Wikipedia
The lipid modified form of LC3, referred to as LC3-II, is believed to be involved in autophagosome membrane expansion and fusion events. [11] However, the exact role of LC3 in the autophagic pathway is still discussed, and the question of whether LC3 is required for autophagy is debated since knockdown of MAP1LC3B is compensated by the other ...
自噬信号通路 | Cell Signaling Technology
脂质化形式的 lc3，也称作 lc3-ii，附着到自噬体的膜上。 自噬与凋亡可以正向也可以负向连接，两种过程之间存在大量的交互作用。 营养缺乏时，自噬功能可看作促生存的机制；然而，过度自噬可导致细胞死亡，这与凋亡的形态不一样。
LC3-positive structures are prominent in autophagy-deficient cells
2019年7月12日 · The lipidated LC3, known as LC3-II, has a faster mobility than LC3-I on SDS PAGE and is relatively specifically associated with autophagosomes and autolysosomes (in the...
How to interpret LC3 immunoblotting - PubMed
Microtubule-associated protein light chain 3 (LC3) is now widely used to monitor autophagy. One approach is to detect LC3 conversion (LC3-I to LC3-II) by immunoblot analysis because the amount of LC3-II is clearly correlated with the number of autophagosomes.
LC3 conjugation system in mammalian autophagy - PMC
2004年8月13日 · LC3 is an autophagosomal ortholog of yeast Atg8. A lipidated form of LC3, LC3-II, has been shown to be an autophagosomal marker in mammals, and has been used to study autophagy in neurodegenerative and neuromuscular …
The regulation, function, and role of lipophagy, a form of ... - Nature
2022年2月8日 · c Lipophagy induces engulfment of small cytoplasmic lipid droplets or large cytoplasmic lipid droplets by LC3-II-positive membranes. Lipid droplets are transported to lysosomes and degraded by ...
An Overview of Autophagy: Morphology, Mechanism, and Regulation
2014年1月1日 · The ATG4-processed form of LC3 is referred to as LC3-I and the PE-conjugated form is called LC3-II . Lipidation of LC3 in mammalian cells is accelerated under conditions of nutrient starvation or other types of stress ( 63 ).
LC3 and Autophagy - SpringerLink
Microtubule-associated protein 1A/1B-light chain 3 (LC3) is a soluble protein with a molecular mass of ∼17 kDa that is distributed ubiquitously in mammalian tissues and cultured cells. During autophagy, autophagosomes engulf cytoplasmic components,...
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