《文献分享·第四期》通过标记基因Lgr5鉴定小肠和结肠干细胞
2020年12月11日 · Lgr5基因（eucine-rich-repeat-containing G-protein-coupled receptor 5, 也称Gpr49)，是从肠道Wnt靶位的诸多基因中挑选出的，只在小肠绒毛的凹陷处（cryp 展开阅读全文
In vitro expansion of single Lgr5 + liver stem cells induced
2013年1月27日 · The Wnt target gene Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5) marks actively dividing stem cells in Wnt-driven, self-renewing tissues such as small intestine and colon 1,...
In vitro expansion of single Lgr5+ liver stem cells induced by Wnt ...
2013年2月14日 · The Wnt target gene Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5) marks actively dividing stem cells in Wnt-driven, self-renewing tissues such as small intestine and colon, stomach and hair follicles. A three-dimensional culture system allows long-term clonal expansion of single …
Identification of stem cells in small intestine and colon by marker ...
2007年10月14日 · Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5, also known as Gpr49) was selected from a panel of intestinal Wnt target genes for its restricted crypt expression. Here, using...
In vitro expansion of single Lgr5+ liver stem cells induced by …
Here we show that Lgr5-LacZ is not expressed in healthy adult liver, yet that small Lgr5-LacZ + cells appear near bile ducts upon damage, coinciding with robust activation of Wnt signaling. As shown by lineage tracing using a novel Lgr5-ires-CreERT2 knock-in allele, damage-induced Lgr5 + cells generate hepatocytes and bile ducts in vivo .
内源性 β-半乳糖苷酶活性标志着 Lgr5-LacZ 和野生 ... - X-MOL
Lgr5-LacZ 小鼠携带编码 β-半乳糖苷酶 (β-gal) 的大肠杆菌 LacZ 基因，该基因在 Lgr5 启动子（一种干细胞/祖细胞标记物）的控制下。 在 Lgr5-LacZ 小鼠受损的肝脏中，表达 β-半乳糖苷酶 (β-gal) 的细胞被认为是潜在的双能肝祖细胞；然而，他们的起源和身份仍然未知。
Leucine-Rich Repeat-Containing G-Protein-Coupled
2010年5月1日 · This strain harbors a lacZ reporter gene just N-terminal to the region of Lgr5 that encodes the first transmembrane domain, essentially creating a null allele. Homozygous disruption of Lgr5 resulted in neonatal lethality, characterized by ingestion of air at birth, resulting in gastrointestinal tract dilation and the absence of milk from the ...
Lgr5 marks cycling, yet long-lived, hair follicle stem cells
2008年10月12日 · In the first, termed Lgr5LacZ, exon 18 of Lgr5 contains an integrated Ires-LacZ/Neo fusion sequence, and in the second, termed Lgr5-EGFP-Ires-CreERT2, the endogenous Lgr5 promoter...
标记基因Lgr5鉴定小肠和结肠干细胞,Nature - X-MOL
2007年10月1日 · Lgr5（富含亮氨酸重复序列的 G 蛋白偶联受体 5，也称为 Gpr49）是从一组肠道 Wnt 靶基因中选出的，因为它限制了隐窝表达。 在这里，使用两个敲入等位基因，我们揭示了 Lgr5 在隐窝底部的循环柱状细胞中的独家表达。
Structure and function of LGR5: An enigmatic G-protein coupled …
Leucine-rich repeat-containing GPCRs contain a characteristic extracellular domain that is an important modulator of intracellular signaling. One member of this class is the leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5), a stem cell marker in intestinal crypts, and mammary glands.