Designed and biologically active protein lattices - Nature
2021年6月17日 · Our study demonstrates that internal voxel design and inter-voxel encoding can be effectively employed to create protein lattices with designed organization, as confirmed by in situ X-ray...
Nucleoprotein - Wikipedia
Nucleoproteins are proteins conjugated with nucleic acids (either DNA or RNA). [1] . Typical nucleoproteins include ribosomes, nucleosomes and viral nucleocapsid proteins. Nucleoproteins tend to be positively charged, facilitating interaction …
Cryo-EM structure of single-layered nucleoprotein-RNA complex …
1 天前 · Among the seven proteins encoded by the MARV genome, the nucleoprotein ... composed of NP protomers not bound to ssRNA and potentially involved in interactions with the VP40 matrix lattice 20.
A Lattice Model for Transcription Factor Access to Nucleosomal …
2010年10月10日 · Because ∼3/4 of the human genomic DNA is found within nucleosomes, their position and DNA interaction is an essential determinant for the DNA access of gene-specific transcription factors and other proteins. Here, a DNA lattice model was developed for describing ligand binding in the presence of a nucleosome.
Self-Assembly of a Designed Nucleoprotein Architecture through ...
2018年11月11日 · We report here a structurally well-defined synthetic nucleoprotein assembly that forms through the synergy of three types of intermolecular interactions: Watson–Crick base pairing, NA–protein interactions, and protein–metal coordination.
Statistical-mechanical lattice models for protein-DNA binding in ...
2010年10月20日 · Statistical-mechanical lattice models for protein-DNA binding are well established as a method to describe complex ligand binding equilibria measured in vitro with purified DNA and protein components. Recently, a new field of applications has opened up for this approach since it has become possible …
Stable Nuclei of Nucleoprotein Filament and High ssDNA …
2017年11月2日 · After DNA end resection, nucleoprotein filament assembly is the key regulatory step in DNA homologous recombination, and it consists of two phases: a rate-limited nucleation step and a fast ...
Mechanical properties of nucleoprotein complexes determined by ...
Here, we describe single-molecule nanoindentation tools that we developed specifically to determine the mechanical properties of histone variant nucleosomes and their complexes.
Structural studies of protein–nucleic acid complexes: A brief …
Cryo-EM was used to determine the 3D structure of the nucleoprotein, but a flexible N-terminal tail was not observed in that structure. To show the flexible parts of the analyzed nucleoprotein, SAXS and CD methods were applied. Modern structural biology poses new challenges that can be achieved by new techniques.
Nucleoprotein - an overview | ScienceDirect Topics
Protein–nucleic acid complexes are defined as conjugates of proteins and nucleic acids, including DNA or RNA or both. Sometimes protein–nucleic acid complexes are also called nucleoproteins; however, it should be emphasized that the term “nucleoproteins” indicates a group of proteins that are associated with nucleic acids.