Oligoribonucleotide - an overview | ScienceDirect Topics
With oligoribonucleotides, the mass differences will be: rC = 305.25, rU = 306.26, rA = 329.27, and rG = 345.27 (all values are atomic weight-based). As is the case for all sequence determination methods that rely on the mass measurements of successive n-mers, oligodeoxynucleotides are easier to characterize due to the relatively large ...
寡核苷酸 - 百度百科
寡核苷酸 (Oligonucleotide)，一般是指2~10核苷酸残基以磷酸二酯键连接而成的线性多核苷酸片段，但在使用这一术语时，对核苷酸残基的数目并无严格规定，在不少文献中，把含有30甚至更多核苷酸残基的多核苷酸分子也称作寡核苷酸。 寡核苷酸可由仪器自动合成，它可作为DNA合成的引物 (Primer)、基因探针 (Probe)等，在现代分子生物学研究中具有广泛的用途。
Oligoribonucleotide - an overview | ScienceDirect Topics
Oligoribonucleotide refers to a short sequence of ribonucleotides that is synthesized using specific chemical procedures and purified for further use. You might find these chapters and articles relevant to this topic. Ryszard Kierzek, in Methods in Enzymology, 2001.
Oligoribonuclease is an essential component of the mRNA decay …
1999年4月4日 · Oligoribonuclease originally was identified as an RNase specific for small oligoribonucleotides (15). Our laboratory subsequently demonstrated that oligoribonuclease is distinct from the other exoribonucleases known in E. coli and that it is the only one able to degrade small oligoribonucleotides (14).
Oligoribonuclease is an essential component of the mRNA decay ... - PNAS
1999年4月13日 · Based on these data, we propose that oligoribonuclease is an essential component of the mRNA-decay pathway in E. coli and, possibly, in higher organisms as well. Bacterial Strains and Plasmids. All strains used were E. coli K-12 derivatives.
Oligoribonucleotides - MeSH - NCBI - National Center for …
Oligoribonucleotides. A group of ribonucleotides (up to 12) in which the phosphate residues of each ribonucleotide act as bridges in forming diester linkages between the ribose moieties. Year introduced: 1986(1976) PubMed search builder options. Subheadings:
Oligonucleotides - SpringerLink
2019年4月14日 · Oligonucleotides are short chains of single stranded or double stranded nucleotides, either DNA, RNA or a combination and in some cases chemically modified to improve their application as a drug substance.
Synthesis of Oligoribonucleotides Containing a 2′-Amino-5′-S ...
2021年10月10日 · Here, we report the synthesis of oligoribonucleotides containing a photocaged 2′-amino-5′-S-phosphorothiolate linkage (Figure Figure1 1 C*) and determine its cleavage rate versus pH in the presence and absence of the hepatitis delta virus (HDV) ribozyme.
Oligoribonucleotide - an overview | ScienceDirect Topics
Short oligoribonucleotides are often conveniently prepared using automated, solid-phase DNA synthesis machines. Chemically protected ribonucleoside phosphoramidites are sequentially coupled to a protected nucleoside attached at its 3′ end to a solid support such as controlled-pore glass or polystyrene.
Oligonucleotide - Wikipedia
Oligonucleotides are short DNA or RNA molecules, oligomers, that have a wide range of applications in genetic testing, research, and forensics.