Single B cell technologies for monoclonal antibody discovery
In this review, we present and discuss the major techniques available to interrogate antigen-specific single B cells to isolate antigen-specific mAbs, including their main advantages and disadvantages.
Single B cell technologies for monoclonal antibody discovery
2021年12月1日 · The Nojima culture describes the generation of in vitro-induced germinal center B cells (iGC B cells) from murine naïve B cells, generated with a specifically engineered feeder cell line termed 40LB, which constitutively expresses CD40 ligand (CD154) and B cell activating factor (BAFF), together with exogenous recombinant murine IL-4 and IL-21 ...
Single B cell technologies for monoclonal antibody discovery
2021年11月4日 · Monoclonal antibodies (mAbs) are often selected from antigen-specific single B cells derived from different hosts, which are notably short-lived in ex vivo culture conditions and hence, arduous to interrogate.
Single-cell approaches to investigate B cells and antibodies in ...
2020年7月29日 · Common pre-enrichment techniques include the isolation of desired B cells with antigenic tetramers 19,20 and the in vitro culture of single B cells into ASCs to detect antigen...
Effective high-throughput isolation of fully human antibodies ... - Nature
2019年10月7日 · For antigen-specific B-cell isolation (I, days 1 and 2), PBMCs are isolated by density centrifugation from peripheral blood and CD19 + B cells are magnetically enriched before single-cell...
Monoclonal Antibody Generation Using Single B Cell Screening …
2024年7月2日 · Several reports highlight the use of single B cell screening to isolate antibodies from human plasma samples with potent and broad neutralizing activities for applications in treating or protecting against infectious diseases, including for human immunodeficiency virus (HIV) and respiratory syncytial (RSV) .
Discovery and characterization of high-affinity, potent SARS …
2021年10月20日 · In this study, single B cell screening was used to interrogate antibody repertoires of immunized mice and isolate antigen-specific IgG1 + memory B cells. Using these methods, high-affinity,...
2019年11月1日 · In this Review, we focus on these most frequently used technologies based on single B cell retrieval to identify antigen-specific mAbs. This includes the hybridoma technology, single B cell cultures using conventional laboratory equipment, surface staining techniques, single B cell Highlights Monoclonal antibodies (mAbs) are
Single B cell technologies for monoclonal antibody discovery
2021年11月1日 · An alternative efficient approach to generate monoclonal antibodies from humans and immunized animals is single B cell antibody technology, which allows for the rapid isolation of potential...
Exploring Single B-Cell Technologies for Antibody Discovery
2024年4月18日 · Researchers use single B cell technologies to isolate and study individual B cells, leading to the discovery of potent monoclonal antibodies. They continue to be a focal point of scientific investigation, especially due to their pivotal role in the onset of autoimmune disorders and cancers when their development or functionality is impaired.
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