Single B cell technologies for monoclonal antibody discovery
In this review, we present and discuss the major techniques available to interrogate antigen-specific single B cells to isolate antigen-specific mAbs, including their main advantages and disadvantages.
Single B cell technologies for monoclonal antibody discovery
2021年12月1日 · In this review, we present and discuss the major techniques available to interrogate antigen-specific single B cells to isolate antigen-specific mAbs, including their main advantages and disadvantages.
Single B cell technologies for monoclonal antibody discovery
2021年11月4日 · In this review, we present and discuss the major techniques available to interrogate antigen-specific single B cells to isolate antigen-specific mAbs, including their main advantages and disadvantages.
Effective high-throughput isolation of fully human antibodies ... - Nature
2019年10月7日 · For antigen-specific B-cell isolation (I, days 1 and 2), PBMCs are isolated by density centrifugation from peripheral blood and CD19 + B cells are magnetically enriched before single-cell...
Stable long‐term cultures of self‐renewing B cells and their ...
In this review, we summarize our studies to the regulation of the replicative life span, differentiation, and maturation of B cells that led to the development of a platform that uses immortalization of human B cells by in vitro genetic modification for antibody development.
Monoclonal Antibody Generation Using Single B Cell Screening …
2024年7月2日 · Several reports highlight the use of single B cell screening to isolate antibodies from human plasma samples with potent and broad neutralizing activities for applications in treating or protecting against infectious diseases, including for human immunodeficiency virus (HIV) and respiratory syncytial (RSV) .
Single B cell antibody technologies - ScienceDirect
2011年9月1日 · The single B cell antibody approaches harbor the potential to isolate functional mAbs reactive against conformational determinants that are present predominantly in vivo and difficult to emulate in vitro. The strategies straightforwardness, the requirement of relatively few cell numbers and the high efficiency in obtaining specific mAbs in a ...
2019年11月1日 · In this Review, we focus on these most frequently used technologies based on single B cell retrieval to identify antigen-specific mAbs. This includes the hybridoma technology, single B cell cultures using conventional laboratory equipment, surface staining techniques, single B cell Highlights Monoclonal antibodies (mAbs) are
Sequencing and cloning of antigen-specific antibodies from
2016年9月15日 · Here we describe a protocol to sequence and clone the variable antibody region of single antigen-specific mouse memory B cells for antibody production. After purification, antigen-specific...
Exploring Single B-Cell Technologies for Antibody Discovery
2024年4月18日 · Researchers use single B cell technologies to isolate and study individual B cells, leading to the discovery of potent monoclonal antibodies. They continue to be a focal point of scientific investigation, especially due to their pivotal role in the onset of autoimmune disorders and cancers when their development or functionality is impaired.