High-throughput identification and quantification of single …
2022年2月22日 · Here, we develop a method, Barcoding Bacteria for Identification and Quantification (BarBIQ), which classifies single bacterial cells into taxa–named herein cell-based operational taxonomy...
A single bacterial genus maintains root growth in a complex ... - Nature
2020年9月30日 · Experiments using an ecologically realistic 185-member bacterial synthetic community in the root system of Arabidopsis reveal that Variovorax bacteria can influence plant hormone levels to...
High-throughput, single-microbe genomics with strain resolution …
We develop and validate Microbe-seq—a high-throughput single-cell sequencing method with strain resolution—and apply it to the human gut microbiome. Using an integrated microfluidic workflow, we encapsulate tens of thousands of microbes individually into droplets.
Selective single-bacteria extraction based on capture and release …
2022年9月14日 · Bacteria identification at the single-cell level provides a reliable biomarker and pathological information for clinical diagnosis. Nevertheless, biosamples obtained from some body sites cannot...
Advancing massive transcriptional profiling of single bacteria
2023年2月27日 · Single-bacteria RNA-seq is becoming a transformative technology for elucidating bacterial diversity in ecology, microbial communities, and pathogenicity. In a recent report by Ma et al. published in Cell, the authors present BacDrop, a versatile, high-throughput method for capturing the transcriptomes of millions of bacteria.
Label-free detection and identification of single bacteria via ...
2023年5月16日 · This application provides a new label-free, noninvasive and nonionizing testing protocol for the detection of single bacteria. 1. Introduction. Humans are exposed to bacterial pathogens all the time, which are present not only in infected patients, but also in soil, water, wildlife, domestic animals, and food.
Single bacteria identification by Raman spectroscopy
We report on rapid identification of single bacteria using a low-cost, compact, Raman spectroscope. We demonstrate that a 60-s procedure is sufficient to acquire a comprehensive Raman spectrum in the range of 600 to 3300 cm⁻¹. This time includes localization of small bacteria aggregates, alignment o ….
Spatiotemporal dynamics of the proton motive force on single
2024年5月23日 · Techniques such as Nernstian and pH-sensitive dyes as well as genetically encoded pH and voltage sensors have recently enabled quantitative measurements at the single-cell level, pushing forward the nascent field of bacterial electrophysiology (15, 26, 27).
Single Bacteria Studies Using Microfluidics | SpringerLink
2015年1月1日 · The importance of individual heterogeneity within a genetically identical population has become well recognized. However, single bacteria studies have been beset by a number of challenges ranging from single-cell handling to detection. Most of these stem from bacteria’s microscale dimensions and the complexity of their natural environment.
Identification of intracellular bacteria from multiple single-cell …
2024年7月3日 · One increasingly popular approach for studying intracellular or host cell–associated microbes is the analysis of microbial reads from single-cell RNA sequencing (scRNA-seq) in the settings of viral infection or of cancer (17–21).