T-cell clones of uncertain significance are highly prevalent and …
2020年5月13日 · We recently introduced a novel flow cytometry assay to detect T-cell clones in blood and bone marrow, based on the identification of a monophasic T-cell receptor (TCR) β …
Identifying and cloning the T cell receptor - Nature
2022年12月6日 · T cells are able to detect and respond to specific peptide antigens by virtue of the unique T cell receptor (TCR) complexes they express on their cell surface (see image).
T-cell clones of uncertain significance are highly prevalent and …
We recently introduced a novel flow cytometry assay to detect T-cell clones in blood and bone marrow, based on the identification of a monophasic T-cell receptor (TCR) β chain constant …
A Practical Approach to T-Cell Receptor Cloning and Expression
We here present an improved 5′-RACE protocol that represents a fast and reliable way to identify a TcR from 10 5 cells only, making TcR cloning feasible without a priori knowledge of the …
Frontiers | Detecting T-cell clonal expansions and quantifying clone ...
2024年4月2日 · Tracking T cells can provide valuable insights into the survival of cells after hematopoietic stem cell transplantation (HSCT) or cancer treatment response and can indicate …
Rapid cloning of antigen-specific T-cell receptors by ... - Nature
2022年4月7日 · We used the detection-and-cloning system to clone a tumour-antigen-specific TCR from peripheral blood mononuclear cells of healthy donors. TCR cloning by leveraging …
Generation of human T cell clones - PubMed
A prototypic limiting dilution cloning procedure to obtain and maintain in culture peptide-specific CD4 positive T cell clones from peripheral blood is detailed. Methods to generate other types …
T cell clonality assessment: past, present and future
Here we review the conceptual foundations of T cell clonality assays, including T cell ontogeny and T cell receptor structure and function; we also provide an introduction to T cell receptor …
Recruitment of epitope-specific T cell clones with a low-avidity ...
Repetitive pathogen exposure leads to the dominant outgrowth of T cell clones with high T cell receptor (TCR) affinity to the relevant pathogen-associated antigens. However, low-affinity …
How Naive T-Cell Clone Counts Are Shaped By Heterogeneous …
2022年2月17日 · We describe the heterogeneous steady-state population of naive T cells (those that have not yet been antigenically triggered) by using a mean-field model of a regulated birth …